Expertise

NeuClone’s scientific team is highly experienced in cultivating industrially relevant cell lines for production of recombinant proteins and other large molecules such as monoclonal antibodies. Particular expertise relates to:

  • Strong molecular biology skills and understanding of metabolic and cellular signalling pathways
  • Cell line engineering - transfections, selection and cell line isolation; single cell cloning; characterization of cell lines; master and working cell banking.
  • CHO, NS0, HEK, BHK cell lines for monoclonal antibody production and many other therapeutic proteins;
  • Vector design and construction for single chain and multi-chain protein expression;
  • Stable cell line development for high recombinant protein production;
  • Transient expression technology for rapid production of milligram/gram amounts of protein for early R&D and pre-clinical studies.
  • Use of fluorescence activated cell sorter (FACS) for accelerated cell line development.
  • Protein biochemistry - post-translational modifications, protein characterization and small scale purification - ion exchange, affinity and immunoaffinity purification.
  • Process development, large-scale mammalian cell culture including protein-free media development, media design and feeding strategies.